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  • CD39 antibody
  • CD39 antibody
  • CD39 antibody
  • CD39 antibody
  • CD39 antibody

CD39 antibody

Cat.#: 109034

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD39 antibody
  • Documents
  • Description
    CD39 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human liver tissue, human tonsillitis tissue. Positive FC detected in Jurkat cells. Positive IP detected in mouse liver tissue. Positive WB detected in Jurkat cells, HL-60 cells, human liver tissue, mouse brain tissue, mouse liver tissue. Observed molecular weight by Western-blot: 75-95 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ATPDase antibody; CD39 antibody; DKFZp686D194 antibody; DKFZp686I093 antibody; Ecto apyrase antibody; Ecto ATP diphosphohydrolase 1 antibody; Ecto ATPase 1 antibody; Ecto ATPDase 1 antibody; ENTPD1 antibody; FLJ40921 antibody; FLJ40959 antibody; NTPDase 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CD39 recombinant protein (Accession Number: BC047664). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109034(ENTPD1 antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109034(ENTPD1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:109034(ENTPD1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:109034(ENTPD1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:109034(ENTPD1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:109034(ENTPD1 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-ENTPD1 (IP:Catalog No:109034, 4ug; Detection:Catalog No:109034 1:800) with mouse liver tissue lysate 4000ug.

    IP Result of anti-ENTPD1 (IP:Catalog No:109034, 4ug; Detection:Catalog No:109034 1:800) with mouse liver tissue lysate 4000ug.

    1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (Catalog No:109034, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (Catalog No:109034, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 19229-1-AP recognizes both the 3 isoforms and soluble form of CD39 (38-478aa) molecule.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"