CD39 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human liver tissue, human tonsillitis tissue. Positive FC detected in Jurkat cells. Positive IP detected in mouse liver tissue. Positive WB detected in Jurkat cells, HL-60 cells, human liver tissue, mouse brain tissue, mouse liver tissue. Observed molecular weight by Western-blot: 75-95 kDa
ELISA, WB, IHC, FC, IP
Human,Mouse,Rat; other species not tested.
ATPDase antibody; CD39 antibody; DKFZp686D194 antibody; DKFZp686I093 antibody; Ecto apyrase antibody; Ecto ATP diphosphohydrolase 1 antibody; Ecto ATPase 1 antibody; Ecto ATPDase 1 antibody; ENTPD1 antibody; FLJ40921 antibody; FLJ40959 antibody; NTPDase 1 antibody
This antibody was obtained by immunization of CD39 recombinant protein (Accession Number: BC047664). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109034(ENTPD1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109034(ENTPD1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109034(ENTPD1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ENTPD1 (IP:Catalog No:109034, 4ug; Detection:Catalog No:109034 1:800) with mouse liver tissue lysate 4000ug.
1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (Catalog No:109034, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 19229-1-AP recognizes both the 3 isoforms and soluble form of CD39 (38-478aa) molecule.
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