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Product Name
EIF2S1 antibody
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Description
EIF2S1 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells, HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive IP detected in HepG2 cells. Positive WB detected in human stomach tissue, human placenta tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 36kd
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EIF 2 antibody; eIF 2 alpha antibody; EIF 2A antibody; EIF 2alpha antibody; EIF2 antibody; EIF2A antibody; EIF2S1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF2S1 recombinant protein (Accession Number: NM_004094). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:110187(EIF2S1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110187(EIF2S1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using EIF2S1 antibody Catalog No:110187 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-EIF2S1 (IP:Catalog No:110187, 4ug; Detection:Catalog No:110187 1:1000) with HepG2 cells lysate 2400ug.
1X10^6 HepG2 cells were stained with .2ug EIF2S1 antibody (Catalog No:110187, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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References
- Yu H, Zheng L, Xu L. Potent effects of the total saponins from Dioscorea nipponica Makino against streptozotocin-induced type 2 diabetes mellitus in rats. Phytotherapy research : PTR. 29(2):228-40. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"