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Product Name
Anti-eIF2A (2E4F5) Mouse antibody
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Description
eIF2A (2E4F5) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
DmelCG6376 ;Dmel_CG6376 ;drosE2F1 ;E(Sev-CycE)3A ;E(var)3-93E ;E2-promoter binding facto ;E2F 1 ;E2F transcription factor 1 ;E2F-1 ;E2f-PA ;E2f-PB ;E2f-PC ;E2F1 ;E2f1 E2F transcription factor 1 ;E2F1_HUMAN ;Evar(3)164 ;KIAA4009 ;l(3)07172 ;l(3)j3B1 ;l(3) antibody
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Isotype
IgG2b
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Preparation
Antigen: Purified recombinant human eIF2α protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/200
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Validations
Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-eIF2α mouse mAb (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using eIF2α.
Western blot detection of eIF2α in K562,COS7,3T3, C2C12,MCF-7,C6 and Hela cell lysates using eIF2α mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.
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Background
Swiss-Prot Acc.P05198.Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"