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DKK3 antibody

Cat.#: 109984

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Product Information

  • Product Name
    DKK3 antibody
  • Documents
  • Description
    DKK3 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human liver cancer tissue, mouse brain tissue. Positive WB detected in mouse brain tissue, human brain tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 55 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    Dickkopf 3 antibody; Dickkopf related protein 3 antibody; Dkk 3 antibody; DKK3 antibody; hDkk 3 antibody; REIC antibody; RIG antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DKK3 recombinant protein (Accession Number: BC007660). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109984(DKK3 antibody) at dilution of 1:500

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109984(DKK3 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109984(DKK3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109984(DKK3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109984(DKK3 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109984(DKK3 antibody) at dilution of 1:50 (under 40x lens)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109984(DKK3 Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109984(DKK3 Antibody) at dilution of 1:1000

    Immunofluorescent analysis of SH-SY5Y cells using Catalog No:109984(DKK3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of SH-SY5Y cells using Catalog No:109984(DKK3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 HepG2 cells were stained with 0.2ug DKK3 antibody (Catalog No:109984, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug DKK3 antibody (Catalog No:109984, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.
  • References
    • Ueno K, Hirata H, Majid S. Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma. Molecular carcinogenesis. 50(6):449-57. 2011.
    • Ueno K, Hirata H, Shahryari V. microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer. British journal of cancer. 108(8):1659-67. 2013.
    • Li X, Xu Y, Chen Y. SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network. Cancer letters. 336(2):379-89. 2013.
    • Ochiai K, Watanabe M, Azakami D. Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours. Veterinary journal (London, England : 1997). 197(3):769-75. 2013.
    • Zhu Y, Demidov ON, Goh AM, Virshup DM, Lane DP, Bulavin DV. Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging. The Journal of clinical investigation. 124(7):3263-73. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"