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Product Name
Cytokeratin 14 antibody
- Documents
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Description
Cytokeratin 14 Mouse Monoclonal antibody. Positive IHC detected in human cervical cancer tissue, human skin tissue. Positive IF detected in A431 cells. Positive WB detected in A431 cells, mouse skin tissue. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CK 14 antibody; CK14 antibody; Cytokeratin 14 antibody; EBS3 antibody; EBS4 antibody; K14 antibody; keratin 14 antibody; KRT14 antibody; NFJ antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Cytokeratin 14 recombinant protein (Accession Number: NM_000526). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:2000
IHC: 1:50-1:500
IF: 1:20-1:200
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Validations
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:107229(KRT14 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:107229(KRT14 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:107229(KRT14 Antibody) at dilution of 1:500
Immunofluorescent analysis of A431 cells using Catalog No:107229(KRT14 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
eratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"