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Product Name
CLTA antibody
- Documents
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Description
CLTA Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HEK-293 cells. Positive IP detected in mouse brain tissue. Positive IF detected in HeLa cells, HEK-293 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 35-38 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Clathrin light chain A antibody; clathrin antibody; light chain (Lca) antibody; CLTA antibody; LCA antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLTA recombinant protein (Accession Number: NM_001833). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109405(CLTA antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109405(CLTA antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-CLTA (IP:Catalog No:109405, 4ug; Detection:Catalog No:109405 1:800) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:109405(CLTA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
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References
- Medendorp K, Vreede L, van Groningen JJ. The mitotic arrest deficient protein MAD2B interacts with the clathrin light chain A during mitosis. PloS one. 5(11):e15128. 2010.
- Lacruz RS, Brookes SJ, Wen X. Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 28(3):672-87. 2013.
- Zhang J, Zhou W, Lin J. Autophagic lysosomal reformation depends on mTOR reactivation in H2O2-induced autophagy. The international journal of biochemistry & cell biology. 70:76-81. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"