-
Product Name
Cdc37 antibody
- Documents
-
Description
Cdc37 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, A431 cells, COS-7 cells, Jurkat cells. Positive IP detected in HEK-293 cells. Positive IF detected in HeLa cells, A431 cells, HEK-293 cells, MCF-7 cells. Positive IHC detected in human colon cancer tissue. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 45-50 kDa
-
Tested applications
ELISA, WB, FC, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
-
Alternative names
CDC37 antibody; CDC37A antibody; Hsp90 co chaperone Cdc37 antibody; P50CDC37 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Cdc37 recombinant protein (Accession Number: NM_007065). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109103(CDC37 antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:109103(CDC37 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:109103(CDC37 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:109103(CDC37 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 MCF-7 cells were stained with 0.2ug CDC37 antibody (Catalog No:109103, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-CDC37 (IP:Catalog No:109103, 4ug; Detection:Catalog No:109103 1:1000) with HEK-293 cells lysate 3200ug.
-
Background
Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. Cdc37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. Cdc37 up-regulation is a common early event in some localized human cancers. But, the effect of Cdc37 dysregulation in human cells is unclear.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"