Product Name

Anti-CDC37 antibody

Documents

Description

Rabbit polyclonal to CDC37

Tested applications

ELISA, WB, IHC-P, IP

Species reactivity

Mouse CDC37

Alternative names

Cdc37 antibody; CDC37 antibody; p50 antibody; p50Cdc37 antibody; P50CDC37 antibody; p50 antibody; P50CDC37 antibody; p50Cdc37 antibody

Immunogen

Mouse CDC37 (His & GST Tag) recombinant protein

Isotype

Rabbit IgG

Preparation

Produced in rabbits immunized with purified, recombinant Mouse CDC37 (rh CDC37; Q61081; Met1-Ala379). CDC37 specific IgG was purified by Mouse CDC37 affinity chromatography.

Clonality

Polyclonal

Formulation

0.2 μm filtered solution in PBS

Storage instructions

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Applications

WB: 5-10 μg/ml

ELISA:0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CDC37. The detection limit for Mouse CDC37 is < 0.039 ng/well.

IHC-P: 0.05-0.5 μg/ml

IP: 1-4 μg/mg of lysate

Validations

CDC37 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

CDC37 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

CDC37 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation

Background

CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is though to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified to Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.

References

Dai K, et al. (1996) P hysical Interaction of Mammalian CDC37 with CDK4. The journal of biological chemistry. 271: 22030-4.
Pearl LH, et al. (2005) Hsp90 and Cdc37-a chaperone cancer conspiracy. Current opinion in genetics development. 15 (1): 55-61.
Chen GQ, et al. (2002) TNF-Induced Recruitment and Activation of the IKK Complex Require Cdc37 and Hsp90. Molecular cell. 9 (2): 401-10.

Anti-CDC37 antibody

Product Information

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