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Product Name
CD33 antibody
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Description
CD33 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human spleen tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human testis tissue. Observed molecular weight by Western-blot: 67 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
CD33 antibody; CD33 molecule antibody; FLJ00391 antibody; gp67 antibody; p67 antibody; SIGLEC 3 antibody; SIGLEC3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD33 recombinant protein (Accession Number: NM_001772). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109028(CD33 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109028(CD33 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109028(CD33 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CD33 antibody Catalog No:109028 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
CD33, aslo referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 expression is restrict to cells of the hematopoietic system. It is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. This molecular may mediate cell-to-cell adhesion and act as receptor that inhibits the proliferation of normal and leukemic myeloid cells.
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References
- Dong J, Li J, Liu SM. CD33⁺/p-STAT1⁺ double-positive cell as a prognostic factor for stage IIIa gastric cancer. Medical oncology (Northwood, London, England). 30(1):442. 2013.
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