Cart 2

Anti-Cd33 antibody

Cat.#: 175317

Size:

Special Price 241.9 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-Cd33 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Cd33
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    gp6 antibody; gp67 antibody; Sigle antibody; Siglec-3 antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from mouse cd33 101-200/334
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:1μg/Test

  • Validations

    Fig1: Blank control: mouse spleen cells (blue).; Primary Antibody: Rabbit Anti- CD33 antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.; Protocol; The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody ( 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

    Fig1: Blank control: mouse spleen cells (blue).; Primary Antibody: Rabbit Anti- CD33 antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.; Protocol; The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody ( 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

    Fig2: Sample:; A549 Cell (Human) Lysate at 30 ug; Primary: Anti-CD33 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 40/37 kD; Observed band size: 40 kD

    Fig2: Sample:; A549 Cell (Human) Lysate at 30 ug; Primary: Anti-CD33 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 40/37 kD; Observed band size: 40 kD

  • Background
    Q63994

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"