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Product Name
Caspase 1/p20/p10 antibody
- Documents
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Description
Caspase 1/p20/p10 Rabbit Polyclonal antibody. Positive IF detected in C6 cells. Positive WB detected in Jurkat cells, C6 cells, HeLa cells, MCF7 cells, MDA-MB-453s cells, NIH/3T3 cells, Raji cells, RAW264.7 cells. Observed molecular weight by Western-blot: 45-47 kDa,30 kDa,35 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CASP 1 antibody; CASP1 antibody; Caspase 1 antibody; Caspase 1/p20/p10 antibody; Caspase1 antibody; ICE antibody; IL 1 beta converting enzyme antibody; IL 1BC antibody; IL1BC antibody; IL1BCE antibody; Interleukin 1 beta convertase antibody; p10 antibody; p20 antibody; P45 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Caspase 1/p20/p10 recombinant protein (Accession Number: NM_033293). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:108870(CASP1 antibody) at dilution of 1:1000
Immunofluorescent analysis of C6 cells using Catalog No:108870(Caspase 1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20.
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References
- Wu Q, Liu MC, Yang J, Wang JF, Zhu YH. Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation. Applied and environmental microbiology. 82(4):1173-82. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"