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Product Name
Beta Arrestin 2 antibody
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Description
Beta Arrestin 2 Rabbit Polyclonal antibody. Positive IHC detected in human spleen tissue, human brain tissue. Positive IF detected in mouse Retina tissue. Positive IP detected in RAW 264.7 cells. Positive WB detected in RAW 264.7 cells, PC-12 cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ARB2 antibody; ARR2 antibody; ARRB2 antibody; Arrestin beta 2 antibody; arrestin antibody; beta 2 antibody; BARR2 antibody; Beta arrestin 2 antibody; DKFZp686L0365 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Beta Arrestin 2 recombinant protein (Accession Number: NM_004313). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
IF result of anti-ARRB2(Catalog No:117126) in Mouse Retina by Dr. Seongjin Seo. IS(Inner segment) Stain.
RAW264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:117126(Beta Arrestin 2 antibody) at dilution of 1:2000
IP Result of anti-Beta Arrestin 2 (IP:Catalog No:117126, 4ug; Detection:Catalog No:117126 1:1000) with RAW 264.7 cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:117126(Beta Arrestin 2 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:117126(Beta Arrestin 2 Antibody) at dilution of 1:200 (under 40x lens).
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Background
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
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References
- Seitz K, Dürsch V, Harnoš J, Bryja V, Gentzel M, Schambony A. β-Arrestin interacts with the beta/gamma subunits of trimeric G-proteins and dishevelled in the Wnt/Ca(2+) pathway in xenopus gastrulation. PloS one. 9(1):e87132. 2014.
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