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Product Name
ATP6V1E1 antibody
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Description
ATP6V1E1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in human brain tissue, HEK-293 cells, HeLa cells, human liver tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 31kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP6E antibody; ATP6E2 antibody; ATP6V1E antibody; ATP6V1E1 antibody; P31 antibody; V ATPase 31 kDa subunit antibody; V ATPase subunit E 1 antibody; Vma4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP6V1E1 recombinant protein (Accession Number: NM_001696). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-ATP6V1E1 (IP:Catalog No:108311, 3ug; Detection:Catalog No:108311 1:500) with mouse brain tissue lysate 6000ug.
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Background
ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.
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References
- Wong D, Bach H, Sun J, Hmama Z, Av-Gay Y. Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification. Proceedings of the National Academy of Sciences of the United States of America. 108(48):19371-6. 2011.
- Yang LS, Xu XE, Liu XP. iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p. Journal of proteomics. 77:480-91. 2012.
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