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Product Name
ASAH1 antibody
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Description
ASAH1 Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in rat lung tissue, human lung tissue, mouse brain tissue, mouse heart tissue, SH-SY5Y cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 60-65 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Acid ceramidase antibody; Acylsphingosine deacylase antibody; ASAH antibody; ASAH1 antibody; FLJ21558 antibody; FLJ22079 antibody; HSD 33 antibody; PHP antibody; PHP32 antibody; Putative 32 kDa heart protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASAH1 recombinant protein (Accession Number: NM_001127505). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108209(ASAH1 antibody) at dilution of 1:50 (under 40x lens)
rat lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:108209(ASAH1 Antibody) at dilution of 1:600
IP Result of anti-ASAH1 (IP:Catalog No:108209, 4ug; Detection:Catalog No:108209 1:300) with mouse brain tissue lysate 3200ug.
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:108209(ASAH1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. It is a 32 kDa heart protein. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.
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References
- Yoshida N, Sawada E, Imokawa G. A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum. Archives of dermatological research. 304(7):563-77. 2012.
- Sawada E, Yoshida N, Sugiura A, Imokawa G. Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis. Journal of dermatological science. 68(1):25-35. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"