ARAF antibody

Cat.#: 108235

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Product Information

  • Product Name
    ARAF antibody
  • Documents
  • Description
    ARAF Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue, human breast cancer tissue. Positive WB detected in HeLa cells, A431 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 33 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    A RAF antibody; ARAF antibody; ARAF1 antibody; PKS antibody; PKS2 antibody; Proto oncogene A Raf antibody; Proto oncogene A Raf 1 antibody; Proto oncogene Pks antibody; RAFA1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ARAF recombinant protein (Accession Number: NM_001256196). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108235(ARAF Antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108235(ARAF Antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using Catalog No:108235(ARAF Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using Catalog No:108235(ARAF Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using Catalog No:108235(ARAF Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using Catalog No:108235(ARAF Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"