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Product Name
Anti-WDR77 (8A10) Mouse antibody
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Description
WDR77 (8A10) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
2610312E17Rik;Androgen receptor cofactor p44;C79984;HKMT1069;MEP 50;MEP-50;MEP50; MEP50_HUMAN;Methylosome protein 50;MGC2722;Nbla10071;p44;p44/Mep50;RGD1310479;RP11 552M11.3;WD repeat containing protein 77;WD repeat domain 77;WD repeat-containing protein antibody
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Isotype
IgG1
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Preparation
Antigen: Purified recombinant human WDR77 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
0.02% NaN3 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/100
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Validations
Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100,next using WDR77 mouse mAb (dilution 1:100).
Western blot detection of WDR77 in C6,3T3 and K562 cell lysates using WDR77 mouse mAb (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
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Background
Swiss-Prot Acc.Q9BQA1.The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.
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