Anti-SAE1 Rabbit antibody

Cat.#: 168885

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Product Information

  • Product Name
    Anti-SAE1 Rabbit antibody
  • Documents
  • Description
    SAE1 Rabbit monoclonal antibody
  • Tested applications
    WB, ICC/IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    AOS1; SUA1; UBLE1A; HSPC140 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human SAE1
  • Clonality
    Monoclonal
  • Formulation
    50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000-1/5000

    ICC/IF: 1/20

  • Validations

    Western blot detection of SAE1 in Hela,Jurkat cell lysates using SAE1 Rabbit mAb(1:500 diluted).Predicted band size:39kDa.Observed band size:39kDa.

    Western blot detection of SAE1 in Hela,Jurkat cell lysates using SAE1 Rabbit mAb(1:500 diluted).Predicted band size:39kDa.Observed band size:39kDa.

  • Background
    Swiss-Prot Acc.Q9UBE0.The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"