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Product Name
Anti-Phospho-p53 (Ser37) Rabbit antibody
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Description
Phospho-p53 (Ser37) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 37 (L-P-(p)S-Q-A) derived from Human p53.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200
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Validations
Western blot detection of p53 (Phospho-Ser37) in 3T3 cells untreated or treated with Alkaline phosphatase using p53 (Phospho-Ser37) Rabbit pAb (dilution 1:1000, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:53kDa.Observed band size:53kDa.
Western blot analysis of extracts from MCF cells untreated or treated with Anisomycin using p53 (Phospho-Ser37) Antibody .
Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser37) Antibody .
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Background
Swiss-Prot Acc.P04637.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"