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Product Name
Anti-Phospho-Histone H2A.X (Ser139) Rabbit antibody
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Description
Phospho-Histone H2A.X (Ser139) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
H2AX; H2A.X; H2A/X antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser139 of human gamma H2A.X
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000
ICC/IF: 1/50
IP: 1/20
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Validations
Western blot detection of gamma H2A.X (Phospho-Ser139) in Jurkat,THP-1,C6,3T3 cell lysates using gamma H2A.X (Phospho-Ser139) Rabbit pAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
Western blot detection of gamma H2A.X (Phospho-Ser139) in HepG2 cell lysates using gamma H2A.X (Phospho-Ser139) Rabbit pAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
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Background
Swiss-Prot Acc.P16104.Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"