Anti-Phospho-FAK (Tyr576/577) Rabbit antibody

Cat.#: 167133

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Product Information

  • Product Name
    Anti-Phospho-FAK (Tyr576/577) Rabbit antibody
  • Documents
  • Description
    Phospho-FAK (Tyr576/577) Rabbit polyclonal antibody
  • Tested applications
    WB, ICC/IF
  • Species reactivity
    Human
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.
  • Clonality
    Polyclonal
  • Formulation
    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB:1/500-1/1000ICC:1/100-1/200

  • Validations

    Western blot detection of FAK(phospho-Tyr576/Tyr577) in Hela cells untreated or UV-treated, using FAK(phospho-Tyr576/Tyr577) Rabbit pAb (dilution 1:1000, upper) or FAK Mouse mAb (200899 dilution 1:500, lower).Predicted band size:125kDa.Observed band size:125kDa.

    Western blot detection of FAK(phospho-Tyr576/Tyr577) in Hela cells untreated or UV-treated, using FAK(phospho-Tyr576/Tyr577) Rabbit pAb (dilution 1:1000, upper) or FAK Mouse mAb (200899 dilution 1:500, lower).Predicted band size:125kDa.Observed band size:125kDa.

    Western blot analysis of extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577) Antibody .

    Western blot analysis of extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577) Antibody .

    Immunofluorescence staining of methanol-fixed Hela cells using FAK (phospho-Tyr576/Tyr577) Antibody .

    Immunofluorescence staining of methanol-fixed Hela cells using FAK (phospho-Tyr576/Tyr577) Antibody .

  • Background
    Swiss-Prot Acc.Q05397.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"