-
Product Name
Anti-p21 Rabbit antibody
- Documents
-
Description
p21 Rabbit polyclonal antibody
-
Tested applications
WB, IHC-P, ICC/IF, IP
-
Species reactivity
Human
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthesized peptide derived from human p21
-
Clonality
Polyclonal
-
Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
-
Validations
Immunofluorescent analysis of MCF7 cells, using p21 Antibody .
Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody.
Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) LnCaP cell lysate.
-
Background
Swiss-Prot Acc.P38936.The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"