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Product Name
Anti-p21 Rabbit antibody
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Description
p21 Rabbit polyclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human p21
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
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Validations
Western blot detection of p21 in MCF-7 cell lysates using p21 Rabbit pAb(1:1000 diluted).Predicted band size:18KDa.Observed band size:21KDa.
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Background
Swiss-Prot Acc.P38936.May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"