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Product Name
Anti-MEK1/2 Rabbit antibody
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Description
MEK1/2 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
CFC3; MEK1; MKK1; MAPKK1; PRKMK1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human MEK1/2
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/5000
IHC: 1/100
ICC/IF: 1/20
FC: 1/20
IP: 1/20
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Validations
Western blot detection of MEK1/2 in K562,C6,3T3,Hela cell lysates using MEK1/2 Rabbit pAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.
Immunocytochemistry of MEK1/2(green) in Hela cells using MEK1/2 Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.P36507.Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"