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Product Name
Anti-MDHA / MDH1 antibody
- Documents
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Description
Rabbit polyclonal to MDHA / MDH1
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Tested applications
ELISA, WB, IP
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Species reactivity
Rat MDHA / MDH1
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Alternative names
B230377B03Rik antibody; D17921 antibody; MDHA antibody; MDHA antibody; Mdhl antibody; MDH-s antibody; MDH-s antibody; MDL1 antibody; MGC:1375 antibody; Mor2 antibody; Mor2 antibody; MOR2 antibody; RP23-198N8.1 antibody; MDHA antibody; MOR2 antibody; MDH-s antibody; HEL-S-32 antibody; MGC:1375 antibody; MDHA antibody; Mor2 antibody; MDH-s antibody; Mor-2 antibody; D17921 antibody; B230377B03Rik antibody; Mor-2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat MDHA / MDH1 (rR MDHA / MDH1; O88989; Met4-Ala334). MDHA / MDH1 specific IgG was purified by Rat MDHA / MDH1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat MDHA / MDH1. The detection limit for Rat MDHA / MDH1 is approximately 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
MDHA / MDH1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
MDHA / MDH1 Antibody, Rabbit PAb, Antigen Affinity Purified
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Background
Malate dehydrogenases 1(MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure.
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References
- Minarik P, et al. (2002) Malate dehydrogenases--structure and function. Gen Physiol Biophys. 21 (3): 257-65.
- Musrati RA, et al. (1998) Malate dehydrogenase: distribution, function and properties. Gen Physiol Biophys. 17 (3): 193-210.
- Hall MD, et al. (1992) Crystal structure of Escherichia coli malate dehydrogenase. A complex of the apoenzyme and citrate at 1.87 A resolution. J Mol Biol. 226 (3): 867-82.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"