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Product Name
Anti-MCM2 (2B5) Mouse antibody
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Description
MCM2 (2B5) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human MCM2 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunofluorescence analysis of Hela cells using MCM2 mouse mAb (green). Blue
Western blot detection of MCM2 in Jurkat,K562,3T3 and Hela cell lysates using MCM2 mouse mAb (1:3000 diluted).Predicted band size:125KDa.Observed band size:125KDa.
Flow cytometric analysis of Jurkat cells using MCM2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.
Immunoprecipitation analysis of Hela cell lysates using MCM2 mouse mAb.
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Background
Swiss-Prot Acc.P49736.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"