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Product Name
Anti-IFNAR1 antibody
- Documents
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Description
Mouse monoclonal to IFNAR1
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Tested applications
ELISA, WB
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Species reactivity
Human IFNAR1 / IFNAR
No cross-reactivity in ELISA with Human cell lysate (293 cell line) -
Alternative names
AVP antibody; AVP antibody; CRF2-1 antibody; CRF2-1 antibody; IFN-alpha-REC antibody; IFN-alpha-REC antibody; IFNAR antibody; IFNAR antibody; IFNAR1 antibody; IFNAR1 antibody; IFNBR antibody; IFNBR antibody; IFN-R-1 antibody; IFN-R-1 antibody; IFRC antibody; IFRC antibody; Ifar antibody; Ifrc antibody; CD118 antibody; Ifnar antibody; Infar antibody; AVP antibody; IFRC antibody; IFNAR antibody; IFNBR antibody; IFN-alpha-REC antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IFNAR1 / IFNAR (rh IFNAR1 / IFNAR; P17181-1; Met 1-Lys 436). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 20-50 μg/ml
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human IFNAR1. The detection limit for Human IFNAR1 is approximately 0.078 ng/well.
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Validations
IFNAR1 / IFNAR Antibody, Mouse MAb, Western blot
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Background
Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.
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References
- Yan H, et al. (1996) Phosphorylated interferon-alpha receptor 1 subunit (IFNaR1) acts as a docking site for the latent form of the 113 kDa STAT2 protein. EMBO J. 15(5): 1064-74.
- Richter MF, et al. (1998) Specific contribution of Tyk2 JH regions to the binding and the expression of the interferon alpha/beta receptor component IFNAR1. J Biol Chem. 273(38): 24723-9.
- Abramovich C, et al. (1997) A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor. EMBO J. 16(2): 260-6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"