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Product Name
Anti-HSP90 (5G4) Mouse antibody
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Description
HSP90 (5G4) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human HSP90AB1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of HSP90AB1 in Hela,PC-12,COS7,C6 and 3T3 cell lysates using HSP90AB1 mouse mAb (1:500 diluted).Predicted band size:90KDa.Observed band size:90KDa.
Immunoprecipitation analysis of Hela cell lysates using Hsp90 mouse mAb.
Immunocytochemistry staining of HeLa cells using HSP90AB1 mouse mAb (dilution 1:100).
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Background
Swiss-Prot Acc.P08238.HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"