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Product Name
Anti-Hsp27 Rabbit antibody
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Description
Hsp27 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Hsp27
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000-1/5000
IHC: 1/20-1/100
ICC/IF: 1/100
FC: 1/20
IP: 1/20
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Validations
Western blot detection of Hsp27 in K562 cell lysates using Hsp27 Rabbit pAb(1:1000 diluted).Predicted band size:23kDa.Observed band size:27kDa.
Immunohistochemistry of Hsp27 in paraffin-embedded Human tonsil using Hsp27 Rabbit pAb at dilution 1/20
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Background
Swiss-Prot Acc.P04792.Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"