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Product Name
Anti-Hsp27 (9G1) Mouse antibody
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Description
Hsp27 (9G1) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human
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Isotype
IgG1
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Preparation
Antigen: Purified recombinant protein expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1;000-2;000
IHC: 1/100-200
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Validations
Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using HSP27 Mouse mAb diluted at 1:200.
Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2,000.
Western blot detection of HSP27 in K562,Hela cell lysates using HSP27 (9G1) Mouse mAb(1:1000 diluted).Predicted band size:27KDa.Observed band size:27KDa.
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Background
Swiss-Prot Acc.P04792.Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"