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Product Name
Anti-HDAC4 (4A3) Mouse antibody
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Description
HDAC4 (4A3) Mouse monoclonal antibody
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat, Monkey
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Alternative names
EC 3.5.1.98;HA6116;HD 4;HD4;HDAC 4;HDAC A;HDAC4;HDAC4_HUMAN;HDACA;Histone Deacetylase 4; Histone Deacetylase A;KIAA0288. antibody
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Isotype
IgG2a
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Preparation
Antigen: Purified recombinant human HDAC4 protein fragments expressed in E.coli
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot detection of HDAC4 in Jurkat,Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted).Predicted band size: 140KDa.Observed band size: 140KDa.
Immunoprecipitation analysis of Hela cell lysates using HDAC4 mouse mAb.
Western blot detection of HDAC4 in C2C12,3T3,K562,Jurkat and Hela cell lysates using HDAC4 mouse mAb(dilution 1:1000).Predicted band size:140kDa.Observed band size:140kDa.
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Background
Swiss-Prot Acc.P56524.Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
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