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Anti-H2BC12 antibody

Cat.#: 176519

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Special Price 258.1 USD

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Product Information

  • Product Name
    Anti-H2BC12 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to H2BC12
  • Tested applications
    WB, ICC/IF, IHC-P
  • Species reactivity
    Human, Mouse
  • Alternative names
    H2BK antibody; H2B/S antibody; H2BFT antibody; H2BFAiii antibody; HIST1H2BK antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000

    ICC/IF: 1:50-1:200

    IHC-P: 1:50-1:200

  • Validations

    Fig1:; ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig1:; ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2:; ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2:; ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3:; ICC staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3:; ICC staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4:; Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

    Fig4:; Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

    Fig5:; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

    Fig5:; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

    Fig6:; Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

    Fig6:; Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

  • Background
  • References
    • Milev MP et al. TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. J Cell Biol 209:221-34 (2015).
    • Hoedt E et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"