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Product Name
Anti-Estrogen Receptor alpha (1G4) Mouse antibody
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Description
Estrogen Receptor alpha (1G4) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human ESR1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using ESR1 mouse mAb with DAB staining.
Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Red
Western blot analysis of extracts from SHSY-5Y and Ramos cell lysates using Estrogen Receptor α mouse mAb (1:500 diluted). Predicted band size:66KDa. Observed band size:66KDa.
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Background
Swiss-Prot Acc.P03372.This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"