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Product Name
Anti-EphB6/Eph Receptor B6 antibody
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Description
Rabbit polyclonal to EphB6/Eph Receptor B6
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Tested applications
ELISA, IHC-P
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Species reactivity
Human EphB6 / Eph Receptor B6
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Alternative names
HEP antibody; Cekl antibody; Cekl antibody; Ephb6 antibody; EphB6 antibody; HEP antibody; Mep antibody; MGC129910 antibody; MGC129911 antibody; MEP antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human EphB6 / Eph Receptor B6 (rh EphB6 / Eph Receptor B6; NP_004436.1; Met1-Ser579). EphB6 / Eph Receptor B6 specific IgG was purified by Human EphB6 / Eph Receptor B6 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human EphB6 / Eph Receptor B6. The detection limit for Human EphB6 / Eph Receptor B6 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
EphB6 / Eph Receptor B6 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human EphB6 in human pancreas with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
EphB6 / Eph Receptor B6 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human EphB6 in human brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. Interestingly, increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration, and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor.
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References
- Munthe E, et al. (2000)Ephrin-B2 is a candidate ligand for the Eph receptor, EphB6. FEBS Lett. 466(1): 169-74.
- Matsuoka H, et al. (2005) Biphasic functions of the kinase-defective Ephb6 receptor in cell adhesion and migration. J Biol Chem. 280(32): 29355-63.
- Truitt L, et al. (2010) The EphB6 receptor cooperates with c-Cbl to regulate the behavior of breast cancer cells. Cancer Res. 70(3): 1141-53.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"