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Product Name
Anti-ECH1 antibody
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Description
Rabbit polyclonal to ECH1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human ECH1
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Alternative names
HPXEL antibody; AA617331 antibody; enoyl CoA hydratase 1 antibody; peroxisomal antibody; ECH1 antibody; HPXEL antibody; delta(3 antibody; 5)-Delta(2 antibody; 4)-dienoyl-CoA isomerase antibody; mitochondrial antibody; delta3 antibody; 5-delta2 antibody; 4-dienoyl-CoA isomerase antibody; enoyl Coenzyme A hydratase 1 antibody; peroxisomal antibody; peroxisomal enoyl-CoA hydratase 1 antibody; enoyl coenzyme A hydratase 1 antibody; peroxisomal antibody; Ech1 antibody; AA617331 antibody; delta(3 antibody; 5)-Delta(2 antibody; 4)-dienoyl-CoA isomerase antibody; mitochondrial antibody; dienoyl-CoA isomerase antibody; peroxisomal/mitochondrial dienoyl-CoA isomerase antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human ECH1 (rh ECH1; Q13011; Thr34-Leu328). ECH1 specific IgG was purified by Human ECH1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ECH1. The detection limit for Human ECH1 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
IP: 0.5-2 μg/mg of lysate
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Background
ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
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References
- Kovalyov LI, et al. (2006) Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry Mosc. 71(4): 448-53.
- Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 127(3):635-48.
- FitzPatrick DR, et al. (1995) Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27(3):457-66.
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