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Product Name
ECH1 antibody
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Description
ECH1 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells, L02 cells, mouse heart tissue. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 30-36kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ECH1 antibody; HPXEL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ECH1 recombinant protein (Accession Number: NM_001398). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110292(ECH1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110292(ECH1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using ECH1 antibody Catalog No:110292 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ECH1 represents enoyl Coenzyme A hydratase 1, peroxisomal
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References
- Ishimoto T, Lanaspa MA, Rivard CJ. High-fat and high-sucrose (western) diet induces steatohepatitis that is dependent on fructokinase. Hepatology (Baltimore, Md.). 58(5):1632-43. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"