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Product Name
Anti-Cyclin B1 Rabbit antibody
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Description
Cyclin B1 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Cyclin B1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000
ICC/IF: 1/20
FC: 1/20
IP: 1/20
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Validations
Immunocytochemistry of Cyclin B1(green) in Hela cells using Cyclin B1 Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of Cyclin B1 in Jurkat cell lysates using Cyclin B1 Rabbit pAb(1:1000 diluted).Predicted band size:48KDa.Observed band size:55KDa.
Western blot detection of Cyclin B1 in K562,Hela cell lysates using Cyclin B1 Rabbit pAb(1:1000 diluted).Predicted band size:48KDa.Observed band size:55KDa.
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Background
Swiss-Prot Acc.P14635.Essential for the control of the cell cycle at the G2/M (mitosis) transition.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"