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Product Name
Anti-CCL2 antibody
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Description
Mouse monoclonal antibody to CCL2
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Tested applications
WB, ICC, IHC-P, FC
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Species reactivity
Human, Mouse, Monkey
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Alternative names
HC11 antibody; MCAF antibody; MCP1 antibody; MCP-1 antibody; SCYA2 antibody; GDCF-2 antibody; SMC-CF antibody; HSMCR30 antibody
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Isotype
IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC-P: 1:100-1:500
FC: 1:100-1:200
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Validations
Fig1: Western blot analysis of CCL2 on human CCL2 recombinant protein using anti-CCL2 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of CCL2 on different cell lysates using anti-CCL2 antibody at 1/1,000 dilution.; Positive control:; Lane 1: A549; Lane 2: HELA; Lane 3: Raw264.7; Lane 4: L1210; Lane 5: C6; Lane 6: Cos7
Fig3: ICC staining CCL2 (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"