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Product Name
Anti-CAPG / AFCP antibody
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Description
Rabbit polyclonal to CAPG / AFCP
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Tested applications
ELISA, WB, IP
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Species reactivity
Human CAPG / AFCP
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Alternative names
capping protein (actin filament) antibody; gelsolin-like antibody; CAPG antibody; AFCP antibody; MCP antibody; actin regulatory protein CAP-G antibody; gelsolin-like capping protein antibody; macrophage capping protein antibody; capping protein (actin filament) antibody; gelsolin-like antibody; Capg antibody; gCap39 antibody; mbh1 antibody; actin regulatory protein CAP-G antibody; actin-capping protein GCAP39 antibody; macrophage-capping protein antibody; myc basic motif homolog 1 antibody; AFCP antibody; HEL-S-66 antibody; mbh1 antibody; gCap39 antibody; MCP antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CAPG / AFCP (rh CAPG / AFCP; AAH14549.1; Met1-Lys348). CAPG / AFCP specific IgG was purified by Human CAPG / AFCP affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CAPG / AFCP. The detection limit for Human CAPG / AFCP is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
CAPG / AFCP Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
CAPG / AFCP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
CAPG, also known as actin Regulatory Protein CAPG, is a a member of the gelsolin/villin family. Members of this family are actin-regulatory proteins. CAPG reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, CAPG contributes to the control of actin-based motility in non-muscle cells. CAPG may also play an important role in macrophage function.
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References
- Watari A. et al., 2007, Oncogene. 25 (56): 7373-80.
- De Corte V. et al., 2005, J Cell Sci. 117 (22): 5283-92.
- Van Impe K. et al., 2003, J Biol Chem. 278 (20): 17945-52.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"