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Product Name
Anti-Alpha Skeletal Muscle Actin Rabbit antibody
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Description
Alpha Skeletal Muscle Actin Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Actin
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/50
ICC/IF: 1/20
FC: 1/20
IP: 1/20
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Validations
Western blot detection of Actin in Jurkat,Ramos,MCF7 cell lysates using Actin Rabbit pAb(1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
Immunohistochemistry of Actin in paraffin-embedded Human colon cancer tissue using Actin Rabbit pAb at dilution 1/100
Western blot detection of Actin in HeLa cell lysates using Actin Rabbit pAb(1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
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Background
Swiss-Prot Acc.P68133.Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"