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Product Name
Anti-Alpha Skeletal Muscle Actin (2B10) Mouse antibody
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Description
Alpha Skeletal Muscle Actin (2B10) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: Synthetic Peptide of α skeletal muscle actin
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-10000
IP:1/200
IF: 1/200
IHC: 1/50-300
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Validations
Western blot detection of Alpha Skeletal Muscle Actin in T47D,3T3,COS7,C6 and Hela cell lysates using Alpha Skeletal Muscle Actin mouse mAb (1:10000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue with diluted at 1:20,000.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,α skeletal muscle actin Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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Background
Swiss-Prot Acc.P68133.Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
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