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pMDLg/pRRE vector (V012789)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012789 pMDLg/pRRE In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev and envelope expressing plasmid

Vector Name:
pMDLg/pRRE
Antibiotic Resistance:
Ampicillin
Length:
8895 bp
Type:
Viral Expression & Packaging Vectors
Replication origin:
ori
Source/Author:
Dr. Didier Trono's lab
Copy Number:
High copy number
Promoter:
CMV+intron
Growth Strain(s):
Stable
Growth Temperature:
37℃
Expression Method:
Constiutive, Stable

pMDLg/pRRE vector Vector Map

pMDLg/pRRE8895 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800CMV enhancerCMV promoterbeta-globin intronHIV-1 gagRREbeta-globin poly(A)oriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMDLg/pRRE vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMDLg-pRRE.gb.        8895 bp DNA     circular SYN 13-DEC-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMDLg-pRRE.gb.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8895)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        161..540
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        541..744
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          878..1353
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1437..2945
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="HIV-1 gag"
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSP
                     TSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALG
                     PGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGK
                     EGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPT
                     APPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2738..5746
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
     misc_feature    5774..6007
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_signal    6238..6631
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(7141..7729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7903..8760)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8761..8865)
                     /label=AmpR promoter