LOCUS       Exported                8890 bp DNA     circular SYN 29-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMDLg-pRRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8890)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        161..540
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        541..744
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          878..1353
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1437..2939
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="HIV-1 gag"
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2732..5740
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5768..6001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_signal    6232..6626
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(7136..7724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7898..8755)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8756..8860)
                     /label=AmpR promoter
ORIGIN
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgaattc gagatctgcc gccgccatgg
     1441 gtgcgagagc gtcagtatta agcgggggag aattagatcg atgggaaaaa attcggttaa
     1501 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1561 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1621 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1681 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1741 acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca
     1801 caggacacag caatcaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa
     1861 tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga
     1921 aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac
     1981 aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa
     2041 aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc
     2101 ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
     2161 cccttcagga acaaatagga tggatgacac ataatccacc tatcccagta ggagaaatct
     2221 ataaaagatg gataatcctg ggattaaata aaatagtaag aatgtatagc cctaccagca
     2281 ttctggacat aagacaagga ccaaaggaac cctttagaga ctatgtagac cgattctata
     2341 aaactctaag agccgagcaa gcttcacaag aggtaaaaaa ttggatgaca gaaaccttgt
     2401 tggtccaaaa tgcgaaccca gattgtaaga ctattttaaa agcattggga ccaggagcga
     2461 cactagaaga aatgatgaca gcatgtcagg gagtgggggg acccggccat aaagcaagag
     2521 ttttggctga agcaatgagc caagtaacaa atccagctac cataatgata cagaaaggca
     2581 attttaggaa ccaaagaaag actgttaagt gtttcaattg tggcaaagaa gggcacatag
     2641 ccaaaaattg cagggcccct aggaaaaagg gctgttggaa atgtggaaag gaaggacacc
     2701 aaatgaaaga ttgtactgag agacaggcta attttttagg gaagatctgg ccttcccaca
     2761 agggaaggcc agggaatttt cttcagagca gaccagagcc aacagcccca ccagaagaga
     2821 gcttcaggtt tggggaagag acaacaactc cctctcagaa gcaggagccg atagacaagg
     2881 aactgtatcc tttagcttcc ctcagatcac tctttggcag cgacccctcg tcacaataaa
     2941 gatagggggg caattaaagg aagctctatt agatacagga gcagatgata cagtattaga
     3001 agaaatgaat ttgccaggaa gatggaaacc aaaaatgata gggggaattg gaggttttat
     3061 caaagtaaga cagtatgatc agatactcat agaaatctgc ggacataaag ctataggtac
     3121 agtattagta ggacctacac ctgtcaacat aattggaaga aatctgttga ctcagattgg
     3181 ctgcacttta aattttccca ttagtcctat tgagactgta ccagtaaaat taaagccagg
     3241 aatggatggc ccaaaagtta aacaatggcc attgacagaa gaaaaaataa aagcattagt
     3301 agaaatttgt acagaaatgg aaaaggaagg aaaaatttca aaaattgggc ctgaaaatcc
     3361 atacaatact ccagtatttg ccataaagaa aaaagacagt actaaatgga gaaaattagt
     3421 agatttcaga gaacttaata agagaactca agatttctgg gaagttcaat taggaatacc
     3481 acatcctgca gggttaaaac agaaaaaatc agtaacagta ctggatgtgg gcgatgcata
     3541 tttttcagtt cccttagata aagacttcag gaagtatact gcatttacca tacctagtat
     3601 aaacaatgag acaccaggga ttagatatca gtacaatgtg cttccacagg gatggaaagg
     3661 atcaccagca atattccagt gtagcatgac aaaaatctta gagcctttta gaaaacaaaa
     3721 tccagacata gtcatctatc aatacatgga tgatttgtat gtaggatctg acttagaaat
     3781 agggcagcat agaacaaaaa tagaggaact gagacaacat ctgttgaggt ggggatttac
     3841 cacaccagac aaaaaacatc agaaagaacc tccattcctt tggatgggtt atgaactcca
     3901 tcctgataaa tggacagtac agcctatagt gctgccagaa aaggacagct ggactgtcaa
     3961 tgacatacag aaattagtgg gaaaattgaa ttgggcaagt cagatttatg cagggattaa
     4021 agtaaggcaa ttatgtaaac ttcttagggg aaccaaagca ctaacagaag tagtaccact
     4081 aacagaagaa gcagagctag aactggcaga aaacagggag attctaaaag aaccggtaca
     4141 tggagtgtat tatgacccat caaaagactt aatagcagaa atacagaagc aggggcaagg
     4201 ccaatggaca tatcaaattt atcaagagcc atttaaaaat ctgaaaacag gaaagtatgc
     4261 aagaatgaag ggtgcccaca ctaatgatgt gaaacaatta acagaggcag tacaaaaaat
     4321 agccacagaa agcatagtaa tatggggaaa gactcctaaa tttaaattac ccatacaaaa
     4381 ggaaacatgg gaagcatggt ggacagagta ttggcaagcc acctggattc ctgagtggga
     4441 gtttgtcaat acccctccct tagtgaagtt atggtaccag ttagagaaag aacccataat
     4501 aggagcagaa actttctatg tagatggggc agccaatagg gaaactaaat taggaaaagc
     4561 aggatatgta actgacagag gaagacaaaa agttgtcccc ctaacggaca caacaaatca
     4621 gaagactgag ttacaagcaa ttcatctagc tttgcaggat tcgggattag aagtaaacat
     4681 agtgacagac tcacaatatg cattgggaat cattcaagca caaccagata agagtgaatc
     4741 agagttagtc agtcaaataa tagagcagtt aataaaaaag gaaaaagtct acctggcatg
     4801 ggtaccagca cacaaaggaa ttggaggaaa tgaacaagta gataaattgg tcagtgctgg
     4861 aatcaggaaa gtactatttt tagatggaat agataaggcc caagaagaac atgagaaata
     4921 tcacagtaat tggagagcaa tggctagtga ttttaaccta ccacctgtag tagcaaaaga
     4981 aatagtagcc agctgtgata aatgtcagct aaaaggggaa gccatgcatg gacaagtaga
     5041 ctgtagccca ggaatatggc agctagattg tacacattta gaaggaaaag ttatcttggt
     5101 agcagttcat gtagccagtg gatatataga agcagaagta attccagcag agacagggca
     5161 agaaacagca tacttcctct taaaattagc aggaagatgg ccagtaaaaa cagtacatac
     5221 agacaatggc agcaatttca ccagtactac agttaaggcc gcctgttggt gggcggggat
     5281 caagcaggaa tttggcattc cctacaatcc ccaaagtcaa ggagtaatag aatctatgaa
     5341 taaagaatta aagaaaatta taggacaggt aagagatcag gctgaacatc ttaagacagc
     5401 agtacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     5461 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     5521 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     5581 gaaaggacca gcaaagctcc tctggaaagg tgaaggggca gtagtaatac aagataatag
     5641 tgacataaaa gtagtgccaa gaagaaaagc aaagatcatc agggattatg gaaaacagat
     5701 ggcaggtgat gattgtgtgg caagtagaca ggatgaggat taacacatgg aattccggag
     5761 cggccgcagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     5821 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     5881 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     5941 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     6001 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     6061 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     6121 gagaaattaa caattacaca agcttccgcg gaattcaccc caccagtgca ggctgcctat
     6181 cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta agctcgcttt
     6241 cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta ctaaactggg
     6301 ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat ttattttcat
     6361 tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg tgggaggtca
     6421 gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa tacactatat
     6481 cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg caacagcccc
     6541 tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt gatttggagg
     6601 ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc tcatgaatgt
     6661 cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact cagttctctt
     6721 gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc gagatggttt
     6781 ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt ctacttgaag
     6841 aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct gcctccccca
     6901 ctcacagtga cccggaatcc ctcgacatgg cagtctagca ctagtgcggc cgcagatctg
     6961 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7021 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7081 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7141 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7201 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7261 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7321 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7381 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7441 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7501 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7561 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7801 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7861 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7921 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7981 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8041 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8101 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8161 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8221 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8281 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8341 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8401 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8461 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8521 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8581 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8641 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8701 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8761 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8821 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8881 cacctgacgt
//
