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pMD2.G vector (V010404)

Price Information

Cat No. Plasmid Name Availability Add to cart
V010404 pMD2.G In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

You may want to buy the packaging plasmid psPAX2 here.

Vector Name:
pMD2.G
Antibiotic Resistance:
Ampicillin
Length:
5822 bp
Type:
Viral Expression & Packaging Vectors
Replication origin:
ori
Source/Author:
Addgene
Copy Number:
High copy number
Promoter:
CMV+intron
Growth Strain(s):
stbl3
Growth Temperature:
37℃

pMD2.G vector Vector Map

pMD2.G5822 bp60012001800240030003600420048005400beta-globin poly(A)oriAmpRAmpR promoterbeta-globin intronVSV-G

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMD2.G vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMD2.G.        5822 bp DNA     circular SYN 26-JAN-2024
DEFINITION  Lentiviral envelope plasmid from the Trono lab.
ACCESSION   .
VERSION     .
KEYWORDS    pMD2.G.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1048..1442
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(1952..2540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2714..3571)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3572..3676)
                     /label=AmpR promoter
     intron          4584..5059
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             join(5134..5822,1..844)
                     /label=VSV-G
                     /note="vesicular stomatitis virus G glycoprotein"