LOCUS       pMD2.G        5822 bp DNA     circular SYN 15-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMD2.G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        59..163
                     /gene="bla"
                     /label=AmpR promoter
     CDS             164..1024
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1195..1783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    2293..2687
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2888..4423)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     intron          4498..4973
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     promoter        complement(5107..5310)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        5311..5690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agcccatggg ggccccctca ggggatccac gtcaggtggc acttttcggg gaaatgtgcg
       61 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      121 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      181 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      241 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      301 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      361 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      421 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      481 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      541 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      601 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      661 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      721 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      781 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      841 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      901 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      961 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1021 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1081 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1141 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1201 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1261 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1321 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1381 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1441 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1501 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1561 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1621 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1681 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1741 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1801 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1861 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1921 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca gatctgcggc cgcactagtg
     1981 ctagactgcc atgtcgaggg attccgggtc actgtgagtg ggggaggcag ggaagaaggg
     2041 ctcacaggac agtcaaacca tgccccctgt ttttccttct tcaagtagac ctctataaga
     2101 caacagagac aactaaggct gagtggccag gcgaggagaa accatctcgc cgtaaaacat
     2161 ggaaggaaca cttcagggga aaggtggtat ctctaagcaa gagaactgag tggagtcaag
     2221 gctgagagat gcaggataag caaatgggta gtgaaaagac attcatgagg acagctaaaa
     2281 caataagtaa tgtaaaatac agcatagcaa aactttaacc tccaaatcaa gcctctactt
     2341 gaatcctttt ctgagggatg aataaggcat aggcatcagg ggctgttgcc aatgtgcatt
     2401 agctgtttgc agcctcacct tctttcatgg agtttaagat atagtgtatt ttcccaaggt
     2461 ttgaactagc tcttcatttc tttatgtttt aaatgcactg acctcccaca ttcccttttt
     2521 agtaaaatat tcagaaataa tttaaataca tcattgcaat gaaaataaat gttttttatt
     2581 aggcagaatc cagatgctca aggcccttca taatatcccc cagtttagta gttggactta
     2641 gggaacaaag gaacctttaa tagaaattgg acagcaagaa agcgagctta gtgatacttg
     2701 tgggccaggg cattagccac accagccacc actttctgat aggcagcctg cactggtggg
     2761 gtgaattccg tttttttttt tttttttcat aaaaattaaa aactcaaata taattgaggc
     2821 ctctttgagc atggtatcac aagttgattt ggtccaaaca tgaagaatct gttgtgcagg
     2881 atttgagtta ctttccaagt cggttcatct ctatgtctgt ataaatctgt cttttcttgg
     2941 tgtgctttaa tttaatgcaa agatggatac caactcggag aaccaagaat agtccaatga
     3001 ttaaccctat gataaagaaa aaagaggcaa tagagctttt ccaactactg aaccaacctt
     3061 ctacaagctc gattggattt ttggatagcc cagtatcacc aaaaaataaa ctctcatcat
     3121 caggaagttg cgaagcagcg tcttgaatgt gaggatgttc gaacacctga gcctttgagc
     3181 taagatgaag atcggagtcc aacataccat gtccaatcat gtataaagga aacttatatc
     3241 ctgaactggt cctcagaact ccattgggtc caatttccac gtcttcatat ggtgcccagt
     3301 catcccacag ttccctttct gtggtagttc cactgatcat tccgaccatt cttgagagga
     3361 ttggagcagc aatatcgact ctgatgtatc tggtctcaaa gtattttagg gtaccattga
     3421 ttatggtgaa agcaggaccg gttcctgggt ttttaggagc aagatagctg agatccactg
     3481 gagagattgg aagacccgct ctgattttgc tccaggtttc ttggcagagg gaataatcca
     3541 agatcctctc aacgtcctga attagactta catccactga ggtctgagat ggagcagaga
     3601 tacttgaccc ttctgggcat tcagggaatc tggctgcagc aaagagatcc ttatcagcca
     3661 tctcgaacca gacacctgat gggagtctga ctccccaatg cttgcagtat tgcattttgc
     3721 aggccttgcc tccagtttca taagcaaagt agttacttct gaaccctgtg ccctcctttc
     3781 ccagggatga tagctctccg tcctctgaga agaaggtgat gtccatggaa atgaggttag
     3841 aatcacatag ccctttgacc ttatagtcag aatgccaggt tgtagagtta tggacagtgg
     3901 ggcatatgta attgctgcat tttccgttga tgaactgtga atcaacccat tctcctgtgt
     3961 attcatcaac cagcacatgg tgaggagtca cctggacaat cactgcttcg gcatccgtca
     4021 cagttgcata tccacaactt tgaggaggga agcctggatt cagccaagtt ccttgtttcg
     4081 tttgttcaat gctttccttg cattgttcta cagatggagt gaaggatcgg atggaatgtg
     4141 ttatatactt cggtccatac cagcggaaat cacaagtagt gacccatttg gaagcatgac
     4201 acatccaacc gtctgcttga atagccttgt gactcttggg cattttgact tgtaaggctg
     4261 tgcctattaa gtcattatgc caatttaaat ctgagcttga cgggcaataa tggtaattag
     4321 aaggaacatt tttccagttt cctttttggt tgtgtggaaa aactatggtg aacttgcaat
     4381 tcaccccaat gaataaaaag gctaagtaca aaaggcactt catagtgtca gaattcagat
     4441 ctcacgtgct ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg
     4501 tgggaggaag ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat
     4561 aatccagcct tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta
     4621 ttagcaatat gaaacctctt acatcagtta caatttatat gcagaaatat ttatatgcag
     4681 aaatattgct attgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag
     4741 aggcatgata cattgtatca ttattgccct gaaagaaaga gattagggaa agtattagaa
     4801 ataagataaa caaaaaagta tattaaaaga agaaagcatt ttttaaaatt acaaatgcaa
     4861 aattaccctg atttggtcaa tatgtgtacc ctgttacttc tccccttcct atgacatgaa
     4921 cttaaccata gaaaagaagg ggaaagaaaa catcaagggt cccatagact caccctgaag
     4981 ttctcaggat ccgagctcgg taccacatgt aagcttcgag gggaggctgg atcggtcccg
     5041 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
     5101 taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     5161 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     5221 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     5281 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     5341 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     5401 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     5461 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     5521 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     5581 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     5641 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacatggcgg
     5701 taatgttgga catgagccaa tataaatgta catattatga tatggataca acgtatgcaa
     5761 tgggccaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccggatcct
     5821 ct
//
