Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V010353 | psPAX2 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
You may want to buy the envelope plasmid pMD2.G here.
psPAX2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
psPAX2 vector Sequence
LOCUS psPAX2. 10668 bp DNA circular SYN 26-JAN-2024
DEFINITION Second generation lentiviral packaging vector from the Trono lab.
ACCESSION .
VERSION .
KEYWORDS psPAX2.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10668)
AUTHORS Addgene
TITLE Direct Submission
COMMENT NGS sequence from Addgene (plasmid #12260).
FEATURES Location/Qualifiers
source 1..10668
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 439..1938
/label=HIV-1 gag
/note="gag protein from human immunodeficiency virus 1"
CDS 1734..4742
/label=HIV-1 pol
/note="pol protein from human immunodeficiency virus 1"
CDS 4839..5012
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate BH5). Accession#: P04612"
misc_feature 5319..5552
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5737..5781
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 5930..5971
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
CDS 6075..6101
/label=NES
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
polyA_signal 6451..6506
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
promoter complement(6915..6945)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6960..6981)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7039..7235
/label=SV40 promoter
/note="SV40 early promoter"
polyA_signal 7241..7375
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(7614..8202)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8376..9233)
/label=AmpR
/note="beta-lactamase"
promoter complement(9234..9338)
/label=AmpR promoter
enhancer 9369..9748
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"