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psPAX2 vector (V010353)

Price Information

Cat No. Plasmid Name Availability Add to cart
V010353 psPAX2 In stock, instant shipping

Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

You may want to buy the envelope plasmid pMD2.G here.

Vector Name:
psPAX2
Antibiotic Resistance:
Ampicillin
Length:
10668 bp
Type:
Viral Expression & Packaging Vectors
Replication origin:
ori
Source/Author:
Addgene
Copy Number:
High copy number

psPAX2 vector Vector Map

psPAX210668 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500HIV-1 gagProtein TatRREgp41 peptideProtein TatNESbeta-globin poly(A) signallac promoterCAP binding siteSV40 promoterSV40 poly(A) signaloriAmpRAmpR promoterCMV enhancer

Plasmid Protocol

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5. Store the plasmid at -20 ℃.

6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it

General Plasmid Transform Protocol

1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.

2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.

3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.

4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.

5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.

psPAX2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V010353                10668 bp    DNA     circular SYN 26-JAN-2024
DEFINITION  Exported.
ACCESSION   V010353
VERSION     V010353
KEYWORDS    psPAX2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Addgene
  TITLE     Direct Submission
COMMENT     NGS sequence from Addgene (plasmid #12260).
FEATURES             Location/Qualifiers
     source          1..10668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             439..1938
                     /label="HIV-1 gag"
                     /note="gag protein from human immunodeficiency virus 1"
     CDS             1734..4742
                     /label="HIV-1 pol"
                     /note="pol protein from human immunodeficiency virus 1"
     CDS             4839..5012
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH5). Accession#: P04612"
                     /label="Protein Tat"
     misc_feature    5319..5552
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5737..5781
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             5930..5971
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             6075..6101
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     polyA_signal    6451..6506
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     promoter        complement(6915..6945)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6960..6981)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7039..7235
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    7241..7375
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7614..8202)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8376..9233)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9234..9338)
                     /label="AmpR promoter"
     enhancer        9369..9748
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"