LOCUS       Exported               10709 bp DNA     circular SYN 26-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10709)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          9870..9910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             439..1941
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1734..4745
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4430..4547
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             4839..5012
                     /codon_start=1
                     /label=Protein Tat
                     /translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALGI
                     SYGRKKRRQRRRA"
     misc_feature    5319..5552
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5688..6347
                     /label=eEnv(Tat/Rev)
     CDS             5737..5781
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             5930..5971
                     /codon_start=1
                     /label=Protein Tat
                     /translation="PTSQPRGDPTGPKE"
     CDS             6075..6101
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     polyA_signal    6451..6506
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6867..6883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6891..6907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6915..6945)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6960..6981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7040..7235
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7086..7221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7241..7375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7614..8202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8373..9233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9234..9338)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9369..9748
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          join(10026..10709,1..333)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
ORIGIN
        1 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
       61 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
      121 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
      181 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
      241 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
      301 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
      361 ctgtctcatc attttggcaa agaattcggg ccgcgttgac gcgcacggca agaggcgagg
      421 ggcggcgact ggtgagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      481 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      541 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
      601 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
      661 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
      721 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaaaaaa
      781 gcacagcaag cagcagctga cacaggacac agcaatcagg tcagccaaaa ttaccctata
      841 gtgcagaaca tccaggggca aatggtacat caggccatat cacctagaac tttaaatgca
      901 tgggtaaaag tagtagaaga gaaggctttc agcccagaag tgatacccat gttttcagca
      961 ttatcagaag gagccacccc acaagattta aacaccatgc taaacacagt ggggggacat
     1021 caagcagcca tgcaaatgtt aaaagagacc atcaatgagg aagctgcaga atgggataga
     1081 gtgcatccag tgcatgcagg gcctattgca ccaggccaga tgagagaacc aaggggaagt
     1141 gacatagcag gaactactag tacccttcag gaacaaatag gatggatgac acataatcca
     1201 cctatcccag taggagaaat ctataaaaga tggataatcc tgggattaaa taaaatagta
     1261 agaatgtata gccctaccag cattctggac ataagacaag gaccaaagga accctttaga
     1321 gactatgtag accgattcta taaaactcta agagccgagc aagcttcaca agaggtaaaa
     1381 aattggatga cagaaacctt gttggtccaa aatgcgaacc cagattgtaa gactatttta
     1441 aaagcattgg gaccaggagc gacactagaa gaaatgatga cagcatgtca gggagtgggg
     1501 ggacccggcc ataaagcaag agttttggct gaagcaatga gccaagtaac aaatccagct
     1561 accataatga tacagaaagg caattttagg aaccaaagaa agactgttaa gtgtttcaat
     1621 tgtggcaaag aagggcacat agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg
     1681 aaatgtggaa aggaaggaca ccaaatgaaa gattgtactg agagacaggc taatttttta
     1741 gggaagatct ggccttccca caagggaagg ccagggaatt ttcttcagag cagaccagag
     1801 ccaacagccc caccagaaga gagcttcagg tttggggaag agacaacaac tccctctcag
     1861 aagcaggagc cgatagacaa ggaactgtat cctttagctt ccctcagatc actctttggc
     1921 agcgacccct cgtcacaata aagatagggg ggcaattaaa ggaagctcta ttagatacag
     1981 gagcagatga tacagtatta gaagaaatga atttgccagg aagatggaaa ccaaaaatga
     2041 tagggggaat tggaggtttt atcaaagtaa gacagtatga tcagatactc atagaaatct
     2101 gcggacataa agctataggt acagtattag taggacctac acctgtcaac ataattggaa
     2161 gaaatctgtt gactcagatt ggctgcactt taaattttcc cattagtcct attgagactg
     2221 taccagtaaa attaaagcca ggaatggatg gcccaaaagt taaacaatgg ccattgacag
     2281 aagaaaaaat aaaagcatta gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt
     2341 caaaaattgg gcctgaaaat ccatacaata ctccagtatt tgccataaag aaaaaagaca
     2401 gtactaaatg gagaaaatta gtagatttca gagaacttaa taagagaact caagatttct
     2461 gggaagttca attaggaata ccacatcctg cagggttaaa acagaaaaaa tcagtaacag
     2521 tactggatgt gggcgatgca tatttttcag ttcccttaga taaagacttc aggaagtata
     2581 ctgcatttac catacctagt ataaacaatg agacaccagg gattagatat cagtacaatg
     2641 tgcttccaca gggatggaaa ggatcaccag caatattcca gtgtagcatg acaaaaatct
     2701 tagagccttt tagaaaacaa aatccagaca tagtcatcta tcaatacatg gatgatttgt
     2761 atgtaggatc tgacttagaa atagggcagc atagaacaaa aatagaggaa ctgagacaac
     2821 atctgttgag gtggggattt accacaccag acaaaaaaca tcagaaagaa cctccattcc
     2881 tttggatggg ttatgaactc catcctgata aatggacagt acagcctata gtgctgccag
     2941 aaaaggacag ctggactgtc aatgacatac agaaattagt gggaaaattg aattgggcaa
     3001 gtcagattta tgcagggatt aaagtaaggc aattatgtaa acttcttagg ggaaccaaag
     3061 cactaacaga agtagtacca ctaacagaag aagcagagct agaactggca gaaaacaggg
     3121 agattctaaa agaaccggta catggagtgt attatgaccc atcaaaagac ttaatagcag
     3181 aaatacagaa gcaggggcaa ggccaatgga catatcaaat ttatcaagag ccatttaaaa
     3241 atctgaaaac aggaaagtat gcaagaatga agggtgccca cactaatgat gtgaaacaat
     3301 taacagaggc agtacaaaaa atagccacag aaagcatagt aatatgggga aagactccta
     3361 aatttaaatt acccatacaa aaggaaacat gggaagcatg gtggacagag tattggcaag
     3421 ccacctggat tcctgagtgg gagtttgtca atacccctcc cttagtgaag ttatggtacc
     3481 agttagagaa agaacccata ataggagcag aaactttcta tgtagatggg gcagccaata
     3541 gggaaactaa attaggaaaa gcaggatatg taactgacag aggaagacaa aaagttgtcc
     3601 ccctaacgga cacaacaaat cagaagactg agttacaagc aattcatcta gctttgcagg
     3661 attcgggatt agaagtaaac atagtgacag actcacaata tgcattggga atcattcaag
     3721 cacaaccaga taagagtgaa tcagagttag tcagtcaaat aatagagcag ttaataaaaa
     3781 aggaaaaagt ctacctggca tgggtaccag cacacaaagg aattggagga aatgaacaag
     3841 tagataaatt ggtcagtgct ggaatcagga aagtactatt tttagatgga atagataagg
     3901 cccaagaaga acatgagaaa tatcacagta attggagagc aatggctagt gattttaacc
     3961 taccacctgt agtagcaaaa gaaatagtag ccagctgtga taaatgtcag ctaaaagggg
     4021 aagccatgca tggacaagta gactgtagcc caggaatatg gcagctagat tgtacacatt
     4081 tagaaggaaa agttatcttg gtagcagttc atgtagccag tggatatata gaagcagaag
     4141 taattccagc agagacaggg caagaaacag catacttcct cttaaaatta gcaggaagat
     4201 ggccagtaaa aacagtacat acagacaatg gcagcaattt caccagtact acagttaagg
     4261 ccgcctgttg gtgggcgggg atcaagcagg aatttggcat tccctacaat ccccaaagtc
     4321 aaggagtaat agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc
     4381 aggctgaaca tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa
     4441 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     4501 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     4561 gggacagcag agatccagtt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg
     4621 cagtagtaat acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca
     4681 tcagggatta tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg
     4741 attaacacat ggaattctgc aacaactgct gtttatccat ttcagaattg ggtgtcgaca
     4801 tagcagaata ggcgttactc gacagaggag agcaagaaat ggagccagta gatcctagac
     4861 tagagccctg gaagcatcca ggaagtcagc ctaaaactgc ttgtaccaat tgctattgta
     4921 aaaagtgttg ctttcattgc caagtttgtt tcatgacaaa agccttaggc atctcctatg
     4981 gcaggaagaa gcggagacag cgacgaagag ctcatcagaa cagtcagact catcaagctt
     5041 ctctatcaaa gcagtaagta gtacatgtaa tgcaacctat aatagtagca atagtagcat
     5101 tagtagtagc aataataata gcaatagttg tgtggtccat agtaatcata gaatatagga
     5161 aaatggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     5221 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     5281 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     5341 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     5401 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     5461 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     5521 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     5581 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     5641 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     5701 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     5761 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     5821 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     5881 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     5941 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     6001 gacagatcca ttcgattagt gaacggatcc ttggcactta tctgggacga tctgcggagc
     6061 ctgtgcctct tcagctacca ccgcttgaga gacttactct tgattgtaac gaggattgtg
     6121 gaacttctgg gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct cctacaatat
     6181 tggagtcagg agctaaagaa tagtgctgtt agcttgctca atgccacagc catagcagta
     6241 gctgagggga cagatagggt tatagaagta gtacaaggag cttgtagagc tattcgccac
     6301 atacctagaa gaataagaca gggcttggaa aggattttgc tataagctcg aaacaaccgg
     6361 tacctctaga actatagcta gcagatcttt ttccctctgc caaaaattat ggggacatca
     6421 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     6481 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     6541 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     6601 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     6661 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     6721 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     6781 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     6841 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6961 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7021 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     7081 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7141 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7201 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     7261 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7321 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     7381 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7441 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7801 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8041 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8221 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8281 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8341 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8401 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8461 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8521 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8581 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8641 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8701 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8761 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8821 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8881 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8941 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9001 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9061 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9121 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9181 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9241 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9301 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9361 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     9421 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     9481 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     9541 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     9601 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     9661 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     9721 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     9781 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     9841 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
     9901 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     9961 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
    10021 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
    10081 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
    10141 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
    10201 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
    10261 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
    10321 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
    10381 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
    10441 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
    10501 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
    10561 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
    10621 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
    10681 ccggcggctg tcgaggcgcg gcgagccgc
//
