Cart 2

Ca and Mg-free Dulbeccos phosphate-buffered saline (CMF-DPBS) Preparation and Recipe

To prepare L  Ca and Mg-free Dulbeccos phosphate-buffered saline (CMF-DPBS)

Table 1. Recipe

Name Amount Concentration Formula/M.W. CAS #
Sodium chloride 8.006 g 0.137 M NaCl/58.44 7647-14-5
Potassium Chloride 0.201 g 2.7 mM KCl/74.55 7447-40-7
1.149 g 8.1 mM Na2HPO4/141.96 7558-79-4
Potassium dihydrogen phosphate 0.20 g 1.47 mM KH2PO4/136.09 7778-77-0
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 8.006 g of Sodium chloride to the solution.
  3. Add 0.201 g of Potassium Chloride to the solution.
  4. Add 1.149 g of Sodium phosphate dibasic to the solution.
  5. Add 0.20 g of Potassium dihydrogen phosphate to the solution.
  6. Add distilled water until the volume is 1 L.