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How much DNA template should be added to a 50ul PCR reaction?

To get good PCR yield and reduce non-specific products, how much DNA template is generally used in a 50ul PCR reaction?

Generally, for low complexity templates (i.e. plasmid, lambda, BAC DNA), use 1 pg–10 ng of DNA per 50 µl reaction.

For higher complexity templates (i.e. genomic DNA), use 1 ng–1 µg of DNA per 50 µl reaction. Usually for small genomes you would use lower amount.

Souce: NovoPro    2018-02-07