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Product Name
Recombinant ZWINT protein (His tag)
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Description
Recombinant Human ZWINT (ZW10-interacting protein 1) fragment (amino acids 1-220) is a high-purity protein designed for structural and biochemical studies. This fragment represents a stable and soluble unit of the outer kinetochore KNL1 complex subunit, essential for investigating chromosome segregation mechanisms and kinetochore-microtubule interactions.
The full-length ZWINT protein acts as a critical component of the outer kinetochore KNL1 complex, which mediates microtubule-kinetochore interactions and serves as a docking platform for spindle assembly checkpoint components. It plays a vital role in chromosome segregation by connecting centromeric DNA to spindle microtubules and ensuring proper alignment during mitosis and meiosis. ZWINT targets the RZZ complex to the kinetochore, recruits MAD2L1, and is essential for activating the spindle assembly checkpoint to correct erroneous kinetochore-microtubule attachments. The protein localizes to the nucleus and specifically to the kinetochore from late prophase to anaphase, highlighting its importance in cell division fidelity.
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Protein name
Outer kinetochore KNL1 complex subunit ZWINT
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Uniprot ID
O95229
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Gene Name
ZWINT
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-220 aa (O95229) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 26.93 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 30 kDa band.
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Purity
>89%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"