Z-DEVD-FMK peptide

Z-DEVD-FMK peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

DEVD, Asp-1 = Z-Asp(OMe), Glu-2 = Glu(OMe), Asp-4 = (OMe)-fluoromethylketone

Benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone

4

95.62%

C₃₀H₄₁FN₄O₁₂

668.67

Synthetic

Powder

Z-DEVD-FMK is a cell-permeable, irreversible inhibitor of caspase-3 with an IC50 of 0.13 µM and no activity at cathepsin B and cathepsin L. Z-DEVD-FMK inhibits apoptotic cell death in many cell types including cancer cells, it is neuroprotective in rat hippocampus following seizures in vivo and local application of Z-DEVD-FMK after spinal cord injury in rats reduces secondary tissue injury and helps preserve motor function.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ma et al (1998) Synergistic effects of caspase inhibitors and MK-801 in brain injury after transient focal cerebral ischaemia in mice. Br J Pharmacol. 124(4) 756 PMID: 9690868
• Henshall et al (2000) Involvement of caspase-3-like protease in the mechanism of cell death following focally evoked limbic seizures. J.Neurochem. 74 1215 PMID: 10693954
• Barut et al (2005) The neuroprotective effects of z-DEVD.fmk, a caspase-3 inhibitor, on traumatic spinal cord injury in rats. Surg Neurol. 64(3) 213 PMID: 16099247

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.